antibody bs352 0295g hrp (Bioss)

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Bioss antibody bs352 0295g hrp
Antibody Bs352 0295g Hrp, supplied by Bioss, used in various techniques. Bioz Stars score: 96/100, based on 417 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bs+0295g+hrp/Goat+Anti-Mouse+IgG+Antibody+(H%2BL)%2C+HRP+Conjugated/pm42106334-46-28-24
Average 96 stars, based on 417 article reviews

antibody bs352 0295g hrp - by Bioz Stars, 2026-09

96/100 stars

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Incubation:

Article Title: MiR-484 Regulates the IL-6/STAT3 Signaling Pathway by Down-Regulating KLF12 to Inhibit the Malignant Progression of Cervical Cancer
Article Snippet: After sealing and washing, the membranes were incubated overnight at 4°C with primary antibodies, including B-cell lymphoma 2 (Bcl-2, ab182858, 1:20,000, Abcam), Bcl-2-associated X protein (ab32503, 1:20,000, Abcam), KLF12 (ab129459, 1:20,000, Abcam), IL-6 (ab233706, 1:20,000, Abcam), Janus Kinase 2 (JAK2, phospho Y1007 + Y1008, ab32101, 1:20,000, Abcam), STAT3 (phospho Y705, ab76315, 1:20,000, Abcam), JAK2 (ab108596, 1:20,000, Abcam), STAT3 (ab68153, 1:20,000, Abcam), and GAPDH (bsm-33033M, 1:50,000, Bioss). .. On the next day, the membranes were incubated with secondary antibodies (bs-0295G-HRP and bs-0296G-HRP, 1:20,000, Bioss), and the protein bands were detected and analyzed using an enhanced chemiluminescent luminescent solution kit (NCM Biotech, China). .. Luciferase reporter gene vectors (Sangon Biotech, China) were constructed with the KLF12 sequence (WT-KLF12) containing miR-1294 binding site and its mutant sequence (MUT-KLF12).

Article Title: Role of copper ion overload triggered by CD44 endocytosis on STAT3-mediated cuproptosis in septic myocardial injury.
Article Snippet: The PVDF membranes were then blocked with 5% skim milk and incubated overnight at 4 ◦C with antibodies targeting CD44 (1:2000, ab238464, abcam, UK), FDX1 (1:1000, A9815, ABclonal, China), LIAS (1:1000, 11577–1-AP, Proteintech, China), Lipoic Acid (1:1000, ab58724, abcam, UK), STAT3 (1:1000, 80149–1-RR, Proteintech, China), β-actin (1:1000, #4967, CST, USA) and p-STAT3 (1:800, #9134, CST, USA). .. Subsequently, a 2-h incubation was carried out with a secondary antibody at a 1:10000 dilution (bs-0295G-HRP, bs-0296G-HRP, Bioss, China). .. The signal was visualized using an enhanced chemiluminescence kit (NCM Biotech, China).

Article Title: Maternal Bisphenol S Exposure Impairs Testicular Development and Sperm Function in Male Offspring by Disrupting the Immune-Endocrine Network.
Article Snippet: Bisphenol S (BPS), a widely used substitute for bisphenol A, is an environmental contaminant with endocrine-disrupting properties.. However, the transgenerational impact of maternal BPS exposure during gestation on male offspring reproductive health and the underlying molecular mechanisms remains unknown.. This study shows that gestational exposure of mice to environmentally relevant concentrations of BPS (3, 30, and 300 μg/kg) doseand time-dependently impairs testicular development and sperm function inmale offspring.

Western Blot:

Article Title: The mechanism of action of GLUT1 in promoting NETs-mediated impairment of macrophage phenotypic switching based on macrophage-fibroblast interplay.
Article Snippet: The Western Protein Marker I (G2086-250UL) was purchased from Servicebio (Wuhan, China). .. The Western Antibody Diluent, glyceraldehyde3-phosphate dehydrogenase (GAPDH)-loading control (bsm-33,033 M), Goat Anti-Rabbit immunoglobulin G (IgG) H&L/horseradish peroxidase (HRP) (bs-0295G-HRP), Goat Anti-Mouse IgG H&L/HRP (bs-0296G- HRP) and the cell counting kit 8 (CCK8) Cell Proliferation Detection Kit came from (Bioss, Beijing, China). .. The mouse HMGB1 enzyme-linked immunosorbent assay (ELISA) Kit, Mouse TNF-α ELISA Kit, and Mouse IL-1β ELISA Kit were obtained from HNYBIO (Shanghai, China).

Article Title: miR-627-5p inhibits malignant progression of cervical cancer by targeting ANGPTL4
Article Snippet: GAPDH , 1:1000 (WB) , bsm-33033M , Bioss (China). .. Goat Anti-Rabbit IgG H&L(HRP) , 1:20000 (WB) , bs-0295G-HRP , Bioss. .. Goat Anti-Mouse IgG H&L(HRP) , 1:20000 (WB) , bs-0296G-HRP , Bioss.

Control:

Cell Counting:

other:

Article Title: NSUN3-Mediated ROS Accumulation Promotes Hepatocellular Carcinoma Proliferation and Activates PI3K/AKT Pathway.
Article Snippet: NSUN3 (NOP2/Sun RNA methyltransferase family member 3) is a gene encoding an RNA methyltransferase primarily responsible for specific methylation modifications on mitochondrial tRNA.. Recent studies have indicated that aberrant expression of NSUN3 may be associated with the development of a range of tumors.. UALCAN and GEPIA2 were utilized for bioinformatics analyses, while lentiviral vectors and small interfering RNA techniques were employed to create cell lines with either overexpression or silencing of NSUN3.

Article Title: PLOD1 promotes gastric cancer metastasis and immune escape by mediating extracellular matrix remodeling
Article Snippet: Goat Anti-Rabbit IgG H&L(HRP) , Bioss , bs-0295G-HRP , 1:20000.

Review